Title
Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast
Description
Abstract Objective The objective of the research presented here was to determine whether autonomous replication sequences (ARS) discovered in the eccDNA replicon of glyphosate resistant Amaranthus palmeri enable self-replication in a yeast system. Results Sequence analysis of the eccDNA replicon revealed a region of sharp changes in A + T/G + C content with characteristic bending indicative of an autonomous replication sequence. Further sequence analysis revealed an extended autonomous replication sequence (EACS) in close proximity to multiple DNA unwinding element (DUE) sequences. This region of the eccDNA replicon enabled autonomous replication of an ARS-less yeast plasmid.
Publication Date
1-1-2020
Publisher
figshare Academic Research System
DOI
10.6084/m9.figshare.c.5056701.v1
Document Type
Data Set
Recommended Citation
Saski, Christopher A.; Molin, William T.; Yaguchi, Allison; Blenner, Mark (2020), "Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast", figshare Academic Research System, doi: 10.6084/m9.figshare.c.5056701.v1
https://doi.org/10.6084/m9.figshare.c.5056701.v1
Identifier
10.6084/m9.figshare.c.5056701.v1
Embargo Date
1-1-2020