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With the progression of regenerative medicine, bio-fillers are becoming a very viable option in the treatment of dental caries and the repair ofcranio-facial injuries.Manyprevious studies have focused onthe use ofbone marrow stem cells (BMSCs) with micro-Hydroxyapatite (HA) particles. However, there have been mixed reports on these particles having positive or negative effects on cells. This work delves into the effects of micro-HA on BMSCs, 7F2 osteoblasts, and dental pulp stem cells (DPSCs) as well as the effects of micro-Fluorapatite (FA) on these cells.ALP and BCA assays were run for 14 days on to determine ALP specific activity and protein content. Antibody stains andconfocalimaging were done for collagenI and osteocalcin. Control cells produced more protein, osteocalcin, and collagen I, and had higher levels ofALP specific activitywith the exception ofa few spikes in each for cells cultured with microparticles.