Date of Award
Master of Science (MS)
M. J. B. Payate
Farrell B. Brown
Single-burst experiments were performed on Bacillus subtilis infected with bacteriophage 29. Viruses were subjected to three doses of UV-irradiation (0, 84, and 750 ergs per mm^2) and then propagated in the host cells at various temperatures (37, 42, or 43.5 C). An average burst size under the control conditions was 135. The data were plotted as histograms so that the distributions of the phage yields could be compared. The Student t-test was applied to compare the burst size distributions for different extents of UV damage and variou growth conditions. The distributions of the burst sizes beame skewed as the conditions were varied. Elevated temperature decreased the burst size of UV-damaged viruses dramatically (71%), but caused a much smaller decrease in the undamage viruses (11%).
Morrow, James R. Jr., "Effects of Temperature and Ultraviolet Radiation on Host-Cell Reactivation of Bacteriophage" (1978). Archived Theses. 94.