Date of Award


Document Type


Degree Name

Master of Science (MS)


Plant and Environmental Science

Committee Chair/Advisor

Eric McLamore

Committee Member

Elizabeth Cieniewicz

Committee Member

Guido Schnabel


Apple stem pitting virus (ASPV) is a latent RNA virus that could cause significant loss to apple production; early-stage field diagnosis could reduce the economic loss caused by ASPV infection. Biosensor is a promising user-friendly detection tool that could transduce biological or chemical interactions into electric signals. Among various types of biosensors, aptasensor, which use aptamers as recognition elements show potential as a high throughput, in-field test. The goal of this thesis is to develop an electrochemical aptasensor testing system for ASPV detection. The quality control study of laser inscribed graphene (LIG) based working electrode of the biosensor was conducted first to improve the reliability of biosensor testing, followed by electrodeposition methods selection and aptamer biofunctionalization and establish electrochemical impedance spectroscopy (EIS) testing system for real leaf samples. This project introduces LIG-based aptasensor for detection of a plant virus, which could be applied to other plant pathogens or human pathogens detection.

Available for download on Friday, May 31, 2024