Transcription profiling by array of normal human and rat bronchial epithelial cells treated with more than 50 different compounds to investigate the ability to translate results from model organisms to humans
Inferring in humans biological responses to external cues such as drugs, chemicals, viruses and hormones, is an essential question in biomedicine and cannot be easily studied in humans. Thus, biomedical research has continuously relied on animal models for studying the impact of these compounds and attempted to “translate” the results to humans. In this context, the Systems Biology Verification for Industrial Methodology for Process Verification in Research (SBV IMPROVER) initiative had run a Species Translation Challenge for the scientific community to explore and understand the limit of translatability from rodent to human using systems biology. Therefore, a multi-layer omics dataset was generated that comprised of phosphoproteomics, transcriptomics and cytokine data derived from normal human (NHBE) and rat (NRBE) bronchial epithelial cells exposed in parallel to more than 50 different stimuli under identical conditions. The present manuscript describes in detail the experimental settings, the generation, processing and quality control analysis of the multi-layer omics dataset. The datasets are accessible in public repositories could be leveraged for further translation studies.
Belcastro, Vincenzo; Meyer, Pablo; Alexopoulos, Leonidas G.; Messinis, Dimitris E.; Rice, John J.; Rhrissorrakrai, Kahn; Melas, Ioannis N; Sakellaropoulos, Theodore; Stolovitzky, Gustavo; Mathis, Carole; Dulize, Rémi H.J.; Bilal, Erhan; Talikka, Marja; Ivanov, Nikolai V.; Norel, Raquel; Boué, Stéphanie; Poussin, Carine (2014), "Transcription profiling by array of normal human and rat bronchial epithelial cells treated with more than 50 different compounds to investigate the ability to translate results from model organisms to humans", ArrayExpress