Data from: Trade-offs between water loss and gas exchange influence habitat suitability of a woodland salamander
heart_rate_dataThe following data was collected from the complementary experiment on the thermal sensitivity of heart rates before and after the exposure to the temperature and humidity treatments.complementary_resid_dataThe following dataset is from the complementary experiment, and it contains the data for the trade-off between skin resistance to water loss and metabolic rate.trade_off_datasetThe following data was used to generate Figure 1 in the publication, and it contains the skin resistance to water loss, volume of oxygen consumption, and the residuals of these traits regressed against mass for every individual across every time point during the acclimation experiment.energy_figure_simuationThese data were generated using our biophysical modeling approach. The mean_energy column represents data that includes the trade-off whereas the mean_J_notrade represents data without the trade-off.activity_figure_simuationThese data were used to generate the activity figure (Fig 4), and it contains estimates of average nightly activity (mean column) over the year assuming conditions are 25% drier than average conditions (roughly what salamanders experience during the driest conditions associated with activity).,1. Reversible acclimation increases resilience to environmental stress, but acclimation may have hidden costs due to underlying links between related physiological traits. Interactions between physiological traits might result in trade-offs that undermine whole-organism performance if the change in a related trait reduces the net benefits of acclimation or increases susceptibility to alternative environmental stressors. 2. Metabolic rate and water loss rate are two fundamental physiological traits that could interact due to their dependence on gas exchange across shared physical surfaces. Reductions in water loss rate in response to dehydration stress might reduce metabolic rate by constraining the flux of both water and oxygen. 3. We examined acclimation of metabolic rate and water loss rate using a species of woodland salamander (Plethodon metcalfi) in response to temperature and humidity using a full factorial experimental design. We controlled the evaporative demand of the air across temperatures to assess temperature and humidity as independent cues for acclimation. We predicted that reductions in water loss rate would coincide with reductions in metabolic rate in response to temperature due to shared physical and chemical pathways. We also assessed acclimation of heart rates as a potential compensatory mechanism used to promote oxygen delivery. We integrated these responses into a biophysical model developed from first principles to demonstrate the potential for these interactions to influence habitat suitability. 4. We found that reductions of water loss rates during thermal acclimation were associated with simultaneous reductions in metabolic rates, and we did not find a compensatory response in heart rates. We suggest that these linkages underlie whole-organism strategies (e.g., physiological dormancy or arousal) for reducing the energetic costs imposed by warm temperatures. The biophysical model suggested that the interaction between these two traits potentially structures phenotypic variation in our population because certain combinations of trait values were incapable of reaching positive energy balance. 5. Trade-offs between linked physiological traits potentially structure whole-organism strategies for responding to environmental stressors and constrain phenotypic variation.
National Science Foundation
Sears, Michael W.; Damm, Jason D.; McPhail, Jared; Riddell, Eric A. (2018), "Data from: Trade-offs between water loss and gas exchange influence habitat suitability of a woodland salamander", DRYAD, doi: 10.5061/dryad.1r3s2