Document Type
Patent
Publication Date
7-10-2001
Patent Number
patent number 6258537
Abstract
The present invention provides a sensitive test for objectively diagnosing the presence of Didymella bryoniae, the causative agent of gummy stem blight, and differentiating it from similar, nonpathogenic Phoma species. The assay is applicable to DNA isolated from extracts from plant leaves, stem or seed. The detection method employs a polymerase chain reaction technique, using specific oligonucleotide primers for amplification. PCR Products can be visualized using an ELISA-based colorimetric detection system.
Application Number
09/255432
Assignees
Clemson University (Clemson, SC)
Filing Date
1999-02-22
Primary/U.S. Class
435/6.12
Other/U.S. Class
435/91.2, 536/22.1, 536/24.3, 536/24.32, 536/25.32
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