Graduate Research and Discovery Symposium (GRADS)


Effects of Local and Global Dynamics on the Supertertiary Organization of Postsynaptic Density Protein 95

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Using a designed network of FRET pairs, we probed interdomain distances between pairwise combinations of subdomains in the full-length postsynaptic density protein 95 (PSD95). In addition to the initial labeling sites, for which helices were chosen, FRET pairs utilizing sites in disordered regions were utilized to probe the dynamics associated with reorientation of these regions that may be absent in the more rigid secondary structural elements. In this study, we used TCSPC to perform single-molecule and “bulk” concentration FRET measurements for each designed mutant of PSD95. Through a global analysis of data from these experiments, we aim to resolve interdomain interactions and their contributions to the supertertiary organization of PSD95. Further, we perform a global analysis of the submillisecond dynamics present to gain insight into the types of motions present for each domain pair.

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