With the current state of progress in trauma management by first responders, there is a need for development of an electrochemical biotransducer that can be used with a wireless implantable biosensor system for continual measurement of interstitial glucose and lactate; beginning from the site of the accident and en-route to a trauma center. Fabrication of oxidoreductase enzyme-rich biorecognition membranes deposited via pyrrole electropolymerization at microfabricated electrodes has been achieved. This construct was then electrochemically overoxidzed to create a non-conductive enzyme-hosting polymer film and finally the entire biotransducer was coated with a phosphorylcholine-containing biomimetic hydrogel to mitigate biofouling and reduce the foreign body response. A catalytic layer of Ni-hexacyanoferrate placed at the electrode-enzyme interface for enhanced peroxide response produced a 20-fold increase (14.19 nA vs. 0.7 nA) in buffered H2O2 measured at 650 mV vs. Ag/AgCl. In vitro characterization showed a sensitivity of 0.68 mA/cm2/mM and 0.36 mA/cm2/mM and a limit of detection of 0.05 mM and 7.9 mM for glucose and lactate respectively.
Karunwi, Olukayode; Alam, Fouzan; and Guiseppi-Elie, Anthony, "Biofabrication and Evaluation, in vitro and in vivo, of a Dual Responsive Glucose and Lactate Implantable Biosensor in a Piglet Trauma Model" (2015). Graduate Research and Discovery Symposium (GRADS). 150.