G01N 30/50 (20130101); B01J 20/28052 (20130101); G01N 33/54353 (20130101); G01N 33/54393 (20130101); B01J 20/281 (20130101); B01D 15/206 (20130101); B01D 15/3823 (20130101); C07K 1/20 (20130101); B01J 20/28023 (20130101); B01J 20/285 (20130101); B01J 20/321 (20130101); B01J 20/3272 (20130101); B01J 20/3274 (20130101); B01J 20/3293 (20130101); Y10T 436/255 (20150115); B01J 2220/56 (20130101); G01N 2030/8813 (20130101)
A solid phase for use in separation has been modified using an aqueous phase adsorption of a headgroup-modified lipid to generate analyte specific surfaces for use as a stationary phase in separations such as high performance liquid chromatography (HPLC) or solid phase extraction (SPE). The aliphatic moiety of the lipid adsorbs strongly to a hydrophobic solid surface, with the hydrophilic and active headgroups orienting themselves toward the more polar mobile phase, thus allowing for interactions with the desired solutes. The surface modification approach is generally applicable to a diversity of selective immobilization applications such as protein immobilization clinical diagnostics and preparative scale HPLC as demonstrated on capillary-channeled fibers, though the general methodology could be implemented on any hydrophobic solid support material.
Clemson University (Clemson, SC)
July 17, 2014