Patent Number 9033159
B01D 71/34 (2013.01) B01D 71/82 (2013.01)
Processes for preparation of macroporous membranes having unusually high equilibrium protein binding capacities are described. Membranes include a self-supporting porous membrane substrate and a grafted polymeric film on the pore surfaces of the substrate. A polymeric film may be grafted to the porous membrane substrate using surface-initiated polymerization. The grafted polymer chains within the polymeric film can act as molecular `brushes` or `tentacles` in solution and can contain one or more capture chemistries for biomolecules. Membranes can be used in the separation and purification of biomolecules such as proteins, nucleic acids, virus or virus-like particles, endotoxins, and the like.
Clemson University, Clemson, SC (US)