Date of Award
Doctor of Philosophy (PhD)
R. Kenneth Marcus, Committee Chair
Jeffrey N. Anker
Philip J. Brown
High performance liquid chromatography (HPLC) is one of the most important and commonly used techniques for protein separation and purification. Stationary phase is the most important component of HPLC since it is where the separation takes place. Traditional HPLC columns that are packed with highly porous micro-particular silica beads suffered from several drawbacks, including high column pressure, low stability of stationary phase, peak broadening and tailing. Large amount of research has been done to develop new HPLC stationary phase for protein separation. Capillary-channeled Polymer (C-CP) fibers have been studied as HPLC stationary phase for protein separation in the Marcus research group for years. C-CP fibers are made from melting extrusion of commonly used polymers such as polypropylene (PP), polyester (PET) and polyamide (Nylon). C-CP fibers are 30 – 50 µm in diameter, with 8 capillary channeled running axially along the whole length of the fibers. These capillary channels largely increase the surface area of C-CP fibers for protein interaction. In this dissertation, different surface modification methods, including physical adsorption of lipid tethered ligands on PP fiber, and chemical modifications of PET and nylon fibers, are studied to enhance the protein separation characteristics of C-CP fiber columns, in terms of protein binding selectivity, capacity, recovery yield as well as the resolution of separation.
Jiang, Liuwei, "Surface Modifications of Capillary-Channeled Polymer (C-CP) Fiber Stationary Phases for Protein Separations" (2016). All Dissertations. 1867.