Fuzzy Supertertiary Interactions within PSD-95 Enable Ligand Binding
Data associated with the publication of the same title for single-molecule FRET experiments used to characterize conformational ensembles of the PSG supramodule of PSD-95. Folder 1. Single Molecule Experimental Data .ht3 and .ptu files corresponding to time-tagged photon records collected using single-photon detectors with Hydra Harp TCSPC software and hardware. Folders are provided for each sample. Selection criteria and correction parameters are detailed in the manuscript. DO data were taken from bursts with SPIE<0.1 while AO data were SPIE>0.9. 1.1 BG Background files from which background signals were calculated for each sample. 1.2 IRF IRF data files used for generation of IRF curves for fluorescence decay histogram analysis and generation of fFCS filters. Fluorescence Decay Histograms Data files for photon counts in each time bin following donor excitation pulse for Donor-only (DO), FRET (DA), and Instrument Response Function (IRF) curves used in fluorescence decay histogram analysis. Filtered FCS Curves and Filter Files Data corresponding to four fFCS curves per sample: low-FRET-high-FRET (lohi), high-FRET-low-FRET (hilo), low-FRET-low-FRET (lolo), and high-FRET-high-FRET (hihi), as well as filter files generated in the Kristine software package using the .ht3 and .ptu data and IRF files from Single Molecule Experimental Data. Simulation AV-Derived Distance Values Structures from DMD simulations and AV-derived interdye distances used for structural classification against experimental FRET data. Also includes references files for associating distances correctly with structures and FRET pairs.
Wu, Fang; Ding, Feng; Seidel, Claus A.M.; Basak, Sujit; Welcome, Franceine S.; Bowen, Mark E.; Saikia, Nabanita; Changcheng, Zhang; Hao, Yan; Hamilton, George; Kubiak, Jakub; Sanabria, Hugo (2022), "Fuzzy Supertertiary Interactions within PSD-95 Enable Ligand Binding", Zenodo, doi: 10.5281/zenodo.6001898