Document Type

Article

Publication Date

6-2016

Publication Title

Biomicrofluidics

Volume

10

Issue

3

Publisher

AIP Publishing

Abstract

Here, we report on an enrichment protocol using carbon electrodedielectrophoresis to isolate and purify a targeted cell population from sample volumes up to 4 ml. We aim at trapping, washing, and recovering an enriched cell fraction that will facilitate downstream analysis. We used an increasingly diluted sample of yeast, 106–102 cells/ml, to demonstrate the isolation and enrichment of few cells at increasing flow rates. A maximum average enrichment of 154.2 ± 23.7 times was achieved when the sample flow rate was 10 μl/min and yeast cells were suspended in low electrically conductive media that maximizes dielectrophoresis trapping. A COMSOL Multiphysics model allowed for the comparison between experimental and simulation results. Discussion is conducted on the discrepancies between such results and how the model can be further improved.

Comments

This manuscript has been published in the journal Biomicrofluidics. Please find the published version here (note that a subscription is necessary to access this version):

http://aip.scitation.org/doi/abs/10.1063/1.4954310?journalCode=bmf

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