Document Type
Patent
Publication Date
6-22-2004
Patent Number
patent number 6753178
Abstract
A plant micropropagation apparatus (100) and process is provided in which a support platform (110) for vessels (12) containing a liquid growth media are periodically pivoted which brings about an intermittent immersion of the plant tissue/growth substrate in the growth media. The motion of the support platform (110) may optionally be used to engage a piston operated dispenser (14) for supplying aseptic media to the sealed vessel (12). The intermittent immersion also provides for an improved method of separating viable embryos from culture materials using a separation matrix in conjunction with the intermittent wave motion of the plant media when suspended in a liquid culture.
Application Number
10/110866
Assignees
Clemson University (Clemson, SC)
Filing Date
2002-04-18
Primary/U.S. Class
435/261
Other/U.S. Class
435/284.1, 435/286.5, 435/286.7, 435/294.1, 435/299.2, 435/308.1