The effect of electric potential on the adsorption of collagen type I onto optically transparent carbon electrodes (OTCE) and its mediation on subsequent adhesion of adult, human, mesenchymal stem cells (hMSCs) is described. Adsorption was investigated as a function of the protein concentration and applied potential. The resulting substrate surfaces were characterized using spectroscopic ellipsometry (SE), atomic force microscopy (AFM), and cyclic voltammetry (CV). While the higher applied potential and protein concentration, the higher the adsorbed amount, the application of potential values higher than +800 mV resulted in the oxidation of the adsorbed protein. Subsequent adhesion of hMSCs on the substrates under standard cell culture conditions was also affected by the potential applied and when the collagen type I was oxidized (under applied potential > +800 mV), hMSCs adhesion was decreased. These results provide the first correlation between the effects of electric potential on protein adsorption and subsequent modulation of anchorage-dependent cell adhesion.
Benavidez, Tomás E.; Wechsler, Marissa E.; Farrer, Madeleine M.; Bizios, Rena; and Garcia, Carlos D., "Electrochemically-Preadsorbed Collagen Promotes Adult Human Mesenchymal Stem Cell Adhesion on Carbon Nanostructured Substrates" (2016). Chemistry Annual Research Symposium. Paper 12.